Journal: Scientific Reports

Article Title: Noscapine targets EGFR p-Tyr1068 to suppress the proliferation and invasion of MG63 cells

doi: 10.1038/srep37062

Figure Lengend Snippet: Noscapine inhibits the growth and the invasion of MG63 and U2OS ( A ) The structure of noscapine. ( B ) Protein level of EGFR in tissues was detected by western blot. ( C ) MRNA level of EGFR in tissues was detected by RT-PCR. *P < 0.05 vs. normal group, **P < 0.01 vs. normal group. ( D ) MG63cells were cultured with indicated concentrations of noscapine for indicated hours in 96-well plates, then MTT assay was performed, results represent the mean ± SD of three experiments done in triplicate. ( E ) MG63cells were pre-incubated with DMSO or noscapine for 24 h, then cells were analyzed using a FACS vantage flow cytometer with the Cell Quest acquisition and analysis software program (Becton Dickinson and Co., San Jose, CA), the experiment was repeated three times. ( F ) MG63cells were pre-incubated with noscapine for 24 h then cells were treated with ANNEXIN-V-FITC apoptosis detection kit and analyzed with FCAS. The experiment was repeated for three independent times. ( G,H ) MG63cells were pre-incubated with noscapine for 24 h; transwell assay without matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. *P < 0.05 vs. DMSO treated group, **P < 0.01 vs. DMSO treated group. ( I,J ) MG63cells were pre-incubated with noscapine for 24 h; transwell assay with matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. *P < 0.05 vs. DMSO treated group, **P < 0.01 vs. DMSO treated group.

Article Snippet: 400 μl of binding buffer was added and the apoptosis rate was examined using BD FACS Vantage flow cytometer (Becton Dickinson).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Cell Culture, MTT Assay, Incubation, Flow Cytometry, Software, Transwell Assay